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antibodies against desmin  (Bioss)


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    Structured Review

    Bioss antibodies against desmin
    Antibodies Against Desmin, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+desmin/Desmin+Polyclonal+Antibody/pm42849581-93-39-44
    Average 93 stars, based on 19 article reviews
    antibodies against desmin - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Efficacy of neonatal mouse muscle extracellular vesicles in skeletal muscle repair and regeneration
    Article Snippet: Equal amounts of protein (20–40 μg) were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, USA). .. After blocking with 5% non-fat milk for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-PAX7 (bs-23668R), anti-MYOD (bs-1378R), anti-eMyHC (bs-70370R), anti-MYOG (bs-1299R), anti-MYHC (bs-9863R), anti-DESMIN (bs-1135R), anti-MF-20 (bs-10985R), anti-ACTIN (bs-0061R), and anti-TUBULIN (bs-4513R) (all from BIOSS, Beijing, China; 1:2000). .. After washing, membranes were incubated with HRP-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (BIOSS, 1:5000) for 1 h at room temperature.

    Incubation:

    Article Title: Efficacy of neonatal mouse muscle extracellular vesicles in skeletal muscle repair and regeneration
    Article Snippet: Equal amounts of protein (20–40 μg) were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, USA). .. After blocking with 5% non-fat milk for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-PAX7 (bs-23668R), anti-MYOD (bs-1378R), anti-eMyHC (bs-70370R), anti-MYOG (bs-1299R), anti-MYHC (bs-9863R), anti-DESMIN (bs-1135R), anti-MF-20 (bs-10985R), anti-ACTIN (bs-0061R), and anti-TUBULIN (bs-4513R) (all from BIOSS, Beijing, China; 1:2000). .. After washing, membranes were incubated with HRP-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (BIOSS, 1:5000) for 1 h at room temperature.

    other:

    Article Title: Effect of octreotide on pancreatic fibrosis in rats with high-fat diet-induced obesity
    Article Snippet: The fasting serum insulin level was measured with an ELISA kit (EZHIASF-14K, EMD Millipore, Billerica, MA, USA).

    Article Title: Effect of octreotide on pancreatic fibrosis in rats with high-fat diet-induced obesity
    Article Snippet: FFA levels were also measured (Randox, Crumlin, UK).

    Immunofluorescence:

    Article Title: circPTPN4 regulates myogenesis via the miR-499-3p / NAMPT axis
    Article Snippet: ProteinFind Goat Anti-Mouse IgG (H + L), HRP Conjugate (HS201–01, 1:1000, TransGen, Beijing, China) and ProteinFind Goat Anti-Rabbit IgG (H + L), HRP Conjugate (HS101–01, 1:500, TransGen, Beijing, China) were used as a secondary antibody. .. Immunofluorescence were performed using anti-Desmin (bs-1026R, 1:100, Bioss, Beijing, China) and anti-MyHC (B103, 2.5 μg/mL, DHSB, Iowa City, IA, USA), as previously described [ ]. .. A fluorescence microscope (DMi8; Leica, Germany) was used to capture three randomly selected fields to visualize the area labeled with anti-MyHC.



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    Image Search Results


    Analysis of MHC and Desmin protein levels during C2C12 myoblast differentiation. Western blot analysis of C2C12 cells cultured on standard (Std) and LS surfaces at different stages of differentiation. Representative immunoblot for (a) MHC and Tubulin, and (c) Desmin and GAPDH. Densitometric analysis of (b) MHC and (d) Desmin protein levels. Data are presented as mean ± SD with individual data points ( n = 3). Statistical significance was assessed using Student's t ‐test ( * p < 0.05; ** p < 0.01; *** p < 0.001 vs. Standard at each time point).

    Journal: Advanced Science

    Article Title: Ultrafast Micrometric Laser Structuring of Polymer Surfaces for Highly Organized Skeletal Muscle Tissue Modeling

    doi: 10.1002/advs.78129

    Figure Lengend Snippet: Analysis of MHC and Desmin protein levels during C2C12 myoblast differentiation. Western blot analysis of C2C12 cells cultured on standard (Std) and LS surfaces at different stages of differentiation. Representative immunoblot for (a) MHC and Tubulin, and (c) Desmin and GAPDH. Densitometric analysis of (b) MHC and (d) Desmin protein levels. Data are presented as mean ± SD with individual data points ( n = 3). Statistical significance was assessed using Student's t ‐test ( * p < 0.05; ** p < 0.01; *** p < 0.001 vs. Standard at each time point).

    Article Snippet: Membranes were blocked with 5% non‐fat dry milk in Tris‐buffered saline containing 0.05% Tween (TBS‐Tween) and then incubated overnight at 4°C with primary antibodies against Myosin Heavy Chain (MHC; 1:1000, Bio‐Techne MAB4470, monoclonal, clone MF20), Desmin (1:3000, Abclonal A3736, monoclonal, clone ARC0235), GAPDH (1:40000, Sigma MAB374, monoclonal, clone 6C5) and Tubulin (1:4000, Sigma T6199, monoclonal, clone DM1A).

    Techniques: Analysis, Western Blot, Cell Culture