Review



desmin  (Bioss)


Bioz Verified Symbol Bioss is a verified supplier
Bioz Manufacturer Symbol Bioss manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Bioss desmin
    Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification <t>(Desmin,</t> <t>Pax7,</t> and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).
    Desmin, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+desmin/Desmin+Polyclonal+Antibody/pmc13120942-51-19-21
    Average 94 stars, based on 8 article reviews
    desmin - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "γ -Aminobutyric acid enhances myogenesis and heat/cold stress resistance in bovine muscle satellite cells"

    Article Title: γ -Aminobutyric acid enhances myogenesis and heat/cold stress resistance in bovine muscle satellite cells

    Journal: Frontiers in Veterinary Science

    doi: 10.3389/fvets.2026.1770540

    Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification (Desmin, Pax7, and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).
    Figure Legend Snippet: Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification (Desmin, Pax7, and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).

    Techniques Used: Activity Assay, Immunofluorescence, Staining, CCK-8 Assay, EdU Assay, Quantitative RT-PCR, Expressing, Western Blot, Control

    Related Articles

    Blocking Assay:

    Article Title: Efficacy of neonatal mouse muscle extracellular vesicles in skeletal muscle repair and regeneration
    Article Snippet: Equal amounts of protein (20–40 μg) were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, USA). .. After blocking with 5% non-fat milk for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-PAX7 (bs-23668R), anti-MYOD (bs-1378R), anti-eMyHC (bs-70370R), anti-MYOG (bs-1299R), anti-MYHC (bs-9863R), anti-DESMIN (bs-1135R), anti-MF-20 (bs-10985R), anti-ACTIN (bs-0061R), and anti-TUBULIN (bs-4513R) (all from BIOSS, Beijing, China; 1:2000). .. After washing, membranes were incubated with HRP-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (BIOSS, 1:5000) for 1 h at room temperature.

    Incubation:

    Article Title: Efficacy of neonatal mouse muscle extracellular vesicles in skeletal muscle repair and regeneration
    Article Snippet: Equal amounts of protein (20–40 μg) were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, USA). .. After blocking with 5% non-fat milk for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-PAX7 (bs-23668R), anti-MYOD (bs-1378R), anti-eMyHC (bs-70370R), anti-MYOG (bs-1299R), anti-MYHC (bs-9863R), anti-DESMIN (bs-1135R), anti-MF-20 (bs-10985R), anti-ACTIN (bs-0061R), and anti-TUBULIN (bs-4513R) (all from BIOSS, Beijing, China; 1:2000). .. After washing, membranes were incubated with HRP-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (BIOSS, 1:5000) for 1 h at room temperature.

    other:

    Article Title: Effect of octreotide on pancreatic fibrosis in rats with high-fat diet-induced obesity
    Article Snippet: The fasting serum insulin level was measured with an ELISA kit (EZHIASF-14K, EMD Millipore, Billerica, MA, USA).

    Article Title: Effect of octreotide on pancreatic fibrosis in rats with high-fat diet-induced obesity
    Article Snippet: FFA levels were also measured (Randox, Crumlin, UK).

    Immunofluorescence:

    Article Title: circPTPN4 regulates myogenesis via the miR-499-3p / NAMPT axis
    Article Snippet: ProteinFind Goat Anti-Mouse IgG (H + L), HRP Conjugate (HS201–01, 1:1000, TransGen, Beijing, China) and ProteinFind Goat Anti-Rabbit IgG (H + L), HRP Conjugate (HS101–01, 1:500, TransGen, Beijing, China) were used as a secondary antibody. .. Immunofluorescence were performed using anti-Desmin (bs-1026R, 1:100, Bioss, Beijing, China) and anti-MyHC (B103, 2.5 μg/mL, DHSB, Iowa City, IA, USA), as previously described [ ]. .. A fluorescence microscope (DMi8; Leica, Germany) was used to capture three randomly selected fields to visualize the area labeled with anti-MyHC.



    Similar Products

    94
    Developmental Studies Hybridoma Bank anti mhc
    Anti Mhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+desmin/anti-Desmin/bio_rxiv__64898__2026__04__26__720818-219-5-6
    Average 94 stars, based on 1 article reviews
    anti mhc - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    92
    Miltenyi Biotec desmin antibody, anti-human, reafinity
    Desmin Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+desmin/Desmin+Antibody%2C+anti-human%2C+REAfinity/custom%40130-119-489%4042502392
    Average 92 stars, based on 1 article reviews
    desmin antibody, anti-human, reafinity - by Bioz Stars, 2026-09
    92/100 stars
      Buy from Supplier

    94
    MedChemExpress monoclonal rabbit anti desmin
    Monoclonal Rabbit Anti Desmin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+desmin/Desmin%2FDES%2C+Human/pm42387573-143-0-8
    Average 94 stars, based on 1 article reviews
    monoclonal rabbit anti desmin - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Bioss desmin
    Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification <t>(Desmin,</t> <t>Pax7,</t> and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).
    Desmin, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+desmin/Desmin+Polyclonal+Antibody/pmc13120942-51-19-21
    Average 94 stars, based on 1 article reviews
    desmin - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    90
    Millipore anti-desmin
    Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification <t>(Desmin,</t> <t>Pax7,</t> and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).
    Anti Desmin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+desmin/desmin+antibody/10__22175_slash_mmb__17760-81-7-11
    Average 90 stars, based on 1 article reviews
    anti-desmin - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    86
    Affinity Biosciences rabbit anti desmin primary antibody
    Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification <t>(Desmin,</t> <t>Pax7,</t> and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).
    Rabbit Anti Desmin Primary Antibody, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+desmin/anti+runx2/pm42314153-89-21-25
    Average 86 stars, based on 1 article reviews
    rabbit anti desmin primary antibody - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    95
    R&D Systems anti desmin
    Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification <t>(Desmin,</t> <t>Pax7,</t> and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).
    Anti Desmin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+desmin/Human%2FMouse+Desmin+Antibody/med_rxiv__64898__2026__04__07__26348559-97-29-30
    Average 95 stars, based on 1 article reviews
    anti desmin - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    86
    Nichirei Corporation desmin
    Transgelin expression pattern in colorectal cancer. (A) Transgelin expression in normal colonic tissues. (B) Transgelin expression in colorectal cancer tissues. (C) Transgelin expression in cancer tissues. (D) Fluorescent immunostaining showed that transgelin was expressed in <t>desmin‐positive</t> smooth muscles as well as in other cancer stromal regions; transgelin, desmin are shown in dark green, green, and purple, respectively. (E) Immunostaining results for transgelin (dark green), smooth muscle <t>Actin</t> <t>(SMA;</t> red), and 4′,6‐diamidino‐2‐phenylindole (DAPI; purple) in the cancer cell stroma. (F) Immunostaining for AE1/3 (green), transgelin (dark green), and DAPI (purple).
    Desmin, supplied by Nichirei Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+desmin/anti+desmin/pmc13062257-146-32-33
    Average 86 stars, based on 1 article reviews
    desmin - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    94
    Thermo Fisher desmin
    Transgelin expression pattern in colorectal cancer. (A) Transgelin expression in normal colonic tissues. (B) Transgelin expression in colorectal cancer tissues. (C) Transgelin expression in cancer tissues. (D) Fluorescent immunostaining showed that transgelin was expressed in <t>desmin‐positive</t> smooth muscles as well as in other cancer stromal regions; transgelin, desmin are shown in dark green, green, and purple, respectively. (E) Immunostaining results for transgelin (dark green), smooth muscle <t>Actin</t> <t>(SMA;</t> red), and 4′,6‐diamidino‐2‐phenylindole (DAPI; purple) in the cancer cell stroma. (F) Immunostaining for AE1/3 (green), transgelin (dark green), and DAPI (purple).
    Desmin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+desmin/D+SALICIN+25GR+25GR/pm41928611-29-50-51
    Average 94 stars, based on 1 article reviews
    desmin - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification (Desmin, Pax7, and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).

    Journal: Frontiers in Veterinary Science

    Article Title: γ -Aminobutyric acid enhances myogenesis and heat/cold stress resistance in bovine muscle satellite cells

    doi: 10.3389/fvets.2026.1770540

    Figure Lengend Snippet: Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification (Desmin, Pax7, and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).

    Article Snippet: Polyclonal antibodies against myogenin (MYOG; BS-3550R, Bioss, Beijing, China), paired box protein 7 (Pax7; AB-528428, Abcam, United Kingdom) and desmin (BS-20702R, Bioss, Beijing, China) were each diluted 1:100 in PBS containing 5% bovine serum albumin (BSA) and incubated overnight at room temperature.

    Techniques: Activity Assay, Immunofluorescence, Staining, CCK-8 Assay, EdU Assay, Quantitative RT-PCR, Expressing, Western Blot, Control

    Transgelin expression pattern in colorectal cancer. (A) Transgelin expression in normal colonic tissues. (B) Transgelin expression in colorectal cancer tissues. (C) Transgelin expression in cancer tissues. (D) Fluorescent immunostaining showed that transgelin was expressed in desmin‐positive smooth muscles as well as in other cancer stromal regions; transgelin, desmin are shown in dark green, green, and purple, respectively. (E) Immunostaining results for transgelin (dark green), smooth muscle Actin (SMA; red), and 4′,6‐diamidino‐2‐phenylindole (DAPI; purple) in the cancer cell stroma. (F) Immunostaining for AE1/3 (green), transgelin (dark green), and DAPI (purple).

    Journal: Cancer Medicine

    Article Title: Transgelin Expression in Activated Cancer‐Associated Fibroblasts Regulates Stromal Contractility and Promotes Colon Cancer Progression

    doi: 10.1002/cam4.71789

    Figure Lengend Snippet: Transgelin expression pattern in colorectal cancer. (A) Transgelin expression in normal colonic tissues. (B) Transgelin expression in colorectal cancer tissues. (C) Transgelin expression in cancer tissues. (D) Fluorescent immunostaining showed that transgelin was expressed in desmin‐positive smooth muscles as well as in other cancer stromal regions; transgelin, desmin are shown in dark green, green, and purple, respectively. (E) Immunostaining results for transgelin (dark green), smooth muscle Actin (SMA; red), and 4′,6‐diamidino‐2‐phenylindole (DAPI; purple) in the cancer cell stroma. (F) Immunostaining for AE1/3 (green), transgelin (dark green), and DAPI (purple).

    Article Snippet: The primary antibodies used were mouse monoclonal anti‐human transgelin antibody (OriGene Technologies, Rockville, MD, USA; used at 1:200 dilution), rabbit monoclonal anti–human α‐SMA (SPRING BIOSCIENCE, Pleasanton, CA, USA; used at 1:200 dilution), Desmin (Nichirei, Tokyo, Japan; used without dilution), 4′,6‐diamidino‐2‐phenylindole (DAPI; Akoya Biosciences, Menlo Park, CA, USA; used without dilution), and AE1/3 antibody (Dako, Agilent Technologies, Santa Clara, CA, USA; used without dilution).

    Techniques: Expressing, Immunostaining, Muscles